11. Uniaxial stretching provides non-uniform deformation field on

the sample [14]. Thus, choosing different parts of the sample

for RNA extraction may lead to different results.

12. The aim of grinding is to obtain a uniform powder without

large sample pieces which can pose an adverse impact on effi-

ciency of RNA extraction in subsequent steps.

13. Count the volume of aqueous phase when transfer.

14. Use PureLink™RNA Mini Kit from this step. Spin cartridge

and collection tube can be found in the kit. Before use, add

ethanol (Molecular Biology grade) to Wash Buffer II as

instructed in product manual.

15. Recovery tube can be found in the PureLink™RNA Mini Kit.

Acknowledgements

The authors acknowledge financial support from Australian

Research Council Industrial Transformation Training Centre for

Personalised Therapeutics Technologies (IC170100016).

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